Antimicrobial activity of nanoemulsion containing Moringa oleifera seed protein
Ngozi Maryann Nebolisa 1 * , Akinwunmi Rapheal Afuape 2 , Aiyebor Augustine Aigbagenode 3 , Elikpi Theresa Onana 4 , Olaiyapo F. Oluwafemi 5
More Detail
1 Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NIGERIA2 Department of Biology, College of Science and Technology, North Carolina Agriculture and Technical State University, Greensboro, NC, USA3 Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, University of Benin, Benin City, NIGERIA4 Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, NIGERIA5 Department of Computational Mathematics, Emory University, Atlanta, GA, USA* Corresponding Author

Abstract

Plant-based antimicrobial substances have been recognized as antimicrobial agents. These peptides demonstrate antimicrobial properties against a wide range of pathogens. This study reports the efficacy of Moringa oleifera nanoemulsion as an antimicrobial. M. oleifera seed was defatted and the protein was extracted from the grounded seed, characterized, and formulated into nanoemulsions by spontaneous nanoemulsification. This method is economically and environmentally safe as the components of nanoemulsion are biodegradable. The formulation was evaluated for particle size, viscosity, pH, antimicrobial activity, and kill time assay. The nanoemulsion was nanosized (43.440 nm - 74.430 nm) with increased encapsulation efficiency in a dose-dependent manner and a suitable pH (5.91 ± 0.01 to 6.14 ± 0.01), excellent dynamic viscosity (32 ± 7 to 39 ± 0). The antimicrobial and minimum inhibitory concentration study displayed a wide range of effectiveness on Pseudomonas aeruginosa and Staphylococcus aureus, the time-kill assay showed moderate biocidal activity. Therefore, M. oleifera seed protein nanoemulsion has the potential to act as antimicrobial.

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

EUR J SUSTAIN DEV RES, Volume 8, Issue 4, 2024, Article No: em0268

https://doi.org/10.29333/ejosdr/15134

Publication date: 01 Oct 2024

Online publication date: 09 Sep 2024

Article Views: 420

Article Downloads: 172

Open Access References How to cite this article