Production of biodiesel by transesterification method from renewable feedstock has come to stay. Biodiesel usage in internal combustion engines is gradually gaining ground due to its ability to produce less carbon (iv) oxide gas than fossil diesel during combustion. The production of less carbon (iv) oxide fumes will help to reduce environmental pollution that causes climate change. Jatropha curcas seed oil is a viable renewable feedstock for biodiesel production. Transesterification of Jatropha curcas seed oil with methanol using solid calcium oxide as catalyst was carried out. The free fatty acid of the oil used was 1.4%, while the molar ratio of methanol to oil, reaction temperature and time were 8:1, 65 °C, and 1hour respectively. The biodiesel produced was analyzed with gas chromatography mass spectrophotometer and the methyl ester content was 87.25%. The fuel properties of the biodiesel produced in a reaction time of about 1 hour 30 minutes are within the range of the values given by ASTM D6751 standard.